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Recombinant Calnexin Rabbit Monoclonal Antibody(ZH5057)
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说明书
规格 价格(¥)
20ul600
50ul1200
100ul2200
反应物种： Human;Mouse;Rat
验证应用： WB;IHC-P;ICC/IF;ELISA;IF-P;IF-Fr
同 种 型： Rabbit IgG
别 名： CANX; CNX; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90; FLJ26570; Calnexin
Pic1

Western blot - Recombinant Calnexin Rabbit Monoclonal Antibody(ZH5057)

Western blot analysis of Calnexin on different lysates. Proteins were transferred to a NC membrane and blocked with 5% NF-Milk in TBST for 1 hour at room temperature. The primary antibody (ZH5057, 1/1000) was used in TBST at room temperature for 2 hours. Goat Anti- Rabbit IgG - HRP Secondary Antibody (ZH1101) at 1:5,000 dilution was used for 1 hour at room temperature.Lane 1: U251 cell Lane 2: Hela cell Lane 3: HepG2 cell Lane 4: K562 cell Lane 5: Raw264.7 cell Lane 6: N-2a cell

Pic2

Immunohistochemistry - Recombinant Calnexin Rabbit Monoclonal Antibody(ZH5057)

Immunohistochemical analysis of paraffin embedded Rat kidney tissue slide using ZH5057(Calnexin antibody) at dilution of 1:200 (under 400x lens). Heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0)

Pic2

Immunohistochemistry - Recombinant Calnexin Rabbit Monoclonal Antibody(ZH5057)

Immunohistochemical analysis of paraffin embedded Mouse kidney tissue slide using ZH5057(Calnexin antibody) at dilution of 1:200 (under 400x lens). Heat mediated antigen retrieval with Sodium citrate buffer (pH 6.0)

Pic3

Immunofluorescence - Recombinant Calnexin Rabbit Monoclonal Antibody(ZH5057)

Immunofluorescent analysis of (4% PFA) fixed HeLa cell using ZH5057(Calnexin antibody) at dilution of 1:50 (under 400x lens).

推荐稀释比
WB 1:500-1:2000
IHC-P 1:100-1:500
ICC/IF 1:50-1:200
IF-P:100-1:500
IF-Fr:100-1:500
ELISA 1:20000
浓度查询
请输入产品标签上的 Lot 号，例如 100625
免疫原

Recombinant protein within human Calnexin. AA range: 1-273.

分子量

Predicted MW: 68 kDa
Observed MW: 90 kDa

克隆号

PC5057

产品形式

liquid

偶联物

Unconjugated

基因ID

821 (Human)12330 (Mouse)29144 (Rat)

UNIPROT ID

P27824 P35564 P35565

存储条件

Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

纯化方式

Affinity-chromatography

WB
WesternBlot实验方案指南
WB
WesternBlot常见问题解析
IHC
免疫组织化学-石蜡切片指南
IHC
免疫组织化学-石蜡切片操作步骤
IHC-P
IHC-P免疫组织化学标准操作流程（石蜡切片——非磷酸化项目检测）
IF/ICC
免疫荧光实验操作流程（细胞样本）
IP
IP检测（磁珠偶联抗体）